Other terms
Probes, DNA,DNA Hybridization Probes,Hybridization Probes, DNA,Probes, DNA Hybridization,Gene Probes, DNA,DNA Gene Probes,Probes, DNA Gene,Chromosomal Probes,Probes, Chromosomal,Whole Genomic DNA Probes,Conserved Gene Probes,Gene Probes, Conserved,Probes, Conserved Gene,Whole Chromosomal Probes,Chromosomal Probes, Whole,Probes, Whole Chromosomal
Description
DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
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